Enzyme Controlled Reactions- (Virtual Lab)
A. ProblemThe purpose of this lab is to observe how enzymes act on substrates to produce products. We will observe how enzyme activity is altered when the enzyme environment's pH is changed.
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C. Materials
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B. Hypothesis
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D. Procedure
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E. ResultsDuring this lab, I observed that as the pH increased the more products were formed. As more substrate was added the number of molecules increased. I also observed that Trial 4 and Trial 5 have the same number of molecules formed, this is because in Trial 5 there are no more substrates. After gathering all the data, and making a graph, I came to the conclusion that the optimum pH level for this lactase-catalyzed reaction is pH of 7 because it shows the highest rate, and it is a neutral pH.
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Number of Molecules Formed per Minute in Varying Levels of pH |
F. Conclusion
Virtual Lab> Journal Questions
After this lab, I concluded that the best pH level for this lactase-catalyzed reaction is in a pH of 7 which is the neutral pH. As it started in the pH of 3 it kept increasing and in the pH of 7 was when it reached the highest level then as it continued to pH 9 it decreased again, making the pH of 7 the highest one. I can conclude that mi hypothesis was almost all the way accurate because as more substrate was added, the number of molecules increase. But then after the pH of 7 the number of molecules formed when down again. And in the pH of 9 and 11 the same number of molecules were formed because there were no more substrates to be added. I would improve this lab next time by maybe changing the ph or adding more substrates to see how the number of molecules created differ. Some experimental errors that cannot be avoided are that maybe you can go a little over the number of substrate added and it can change your whole experiment.
- The initial reaction rate as substrate concentration increases will go up because as you add more substrates, the more products will be made. Trial 4 and Trial 5 are the same because in Trial 5 there are no more substrates.
- Trial 4 and 5 given 4 and 8 grams of lactose.
- The maximum initial reaction cannot be reached at low lactose concentrations because there are not many substrates.
- The data indicates that the optimum pH level for this lactase-catalyzed reaction is pH of 7 because it has the highest rate.
- This suggest that enzymes can be affected by the effects of temperature.
- The producer of this products need to have in mind that the best pH level of the lactase enzyme is a pH of 7 because it is the one that has the highest rate, making it the best one.
After this lab, I concluded that the best pH level for this lactase-catalyzed reaction is in a pH of 7 which is the neutral pH. As it started in the pH of 3 it kept increasing and in the pH of 7 was when it reached the highest level then as it continued to pH 9 it decreased again, making the pH of 7 the highest one. I can conclude that mi hypothesis was almost all the way accurate because as more substrate was added, the number of molecules increase. But then after the pH of 7 the number of molecules formed when down again. And in the pH of 9 and 11 the same number of molecules were formed because there were no more substrates to be added. I would improve this lab next time by maybe changing the ph or adding more substrates to see how the number of molecules created differ. Some experimental errors that cannot be avoided are that maybe you can go a little over the number of substrate added and it can change your whole experiment.